Optimizing Modified mRNA In Vitro Synthesis Protocol for Heart Gene Therapy.

Synthetic modified RNA (modRNA) is a novel vector for gene transfer to the heart and other organs. modRNA can mediate strong, transient protein expression with minimal induction of the innate immune response and risk for genome integration. modRNA is already being used in several human clinical trials, and its use in basic and translational science is growing.

GSA 10

B5767-10 10 mg
EUR 229

SC-10

B6299-10 10 mg
EUR 213

MRT 10

B7685-10 10 mg
EUR 238

Keratin 10 antibody

10-2541 250 ug
EUR 492
Description: Mouse monoclonal Keratin 10 antibody

MFZ 10-7

B5595-10 10 mg
EUR 350

TC ASK 10

B5728-10 10 mg
EUR 438

PSB 10 hydrochloride

B6923-10 10 mg
EUR 366

10-DEBC hydrochloride

B7105-10 10 mg
EUR 221
Description: 10-DEBC hydrochloride is a selective inhibitor of Akt (or termed PKB) [1], with an IC50 value of approximate 48 ?M [2].Akt is a type of serine/threonine kinase. It phosphorylates and inactivates components in the apoptotic machinery, including Caspase 9 and BAD.

AI-10-49

A8694-10 10 mg
EUR 212
Description: AI-10-49 is a selective inhibitor of CBF? -SMMHC and RUNX1 interaction with a FRET IC50 value of 260nM. AI-10-49 restores RUNX1 transcriptional activity, displays favorable pharmacokinetics, and delays leukemia progression in mice.

KinaSorb™ 10

KE785-10 10 preps
EUR 461

IL-10, human recombinant

4155-10
EUR 245

IL-10, murine recombinant

4156-10
EUR 245

IL-10, rat recombinant

4157-10
EUR 300

C-10, murine recombinant

4008-10
EUR 256

HG-10-102-01

B1262-10 10 mg
EUR 303
Description: HG-10-102-01 is a potent and selective inhibitor of leucine-rich repeat kinase 2 (LRRK2) with the IC50 values of 20.3nM and 3.2nM for wild type LRRK2 and LRRK2 [G1019S], respectively [1].

Ro 10-5824 dihydrochloride

B7019-10 10 mg
EUR 267

Individual Reaction Mix 10

G065-10 200 reactions
EUR 167

GFP Antibody, 10 uL

P601-10 NULL
EUR 0

BSA (10% in H?O)

2119-10
EUR 137

7078D 10 PIPET 10ML STERILE

7078D-10 25/pk
EUR 259
Description: Disposable Pipets; Disposable Pipets, Serological, Misc

Beta-Lipotropin (1-10), porcine

A1014-10 10 mg
EUR 166
Description: Morphine-like substances exist in brain or the pituitary of various species. Beta-lipotropin (beta-LPH) was found to contain within its C-terminal sequence the primary structure of these peptides.

Amyloid ?-Peptide (10-20) (human)

A1038-10 10 mg
EUR 630
Description: The amyloid ?-peptide (A?) has a central role in initiating neurodegeneration in Alzheimer disease (AD) 1. It is widely believed to be an incidental catabolic byproduct of the amyloid ? protein precursor (APP) with no normal physiological function.

Amyloid ?-peptide (10-35), amide

A1102-10 10 mg
EUR 456
Description: Amyloid ?-protein (10-35) was used as a trunCated peptide model for the full-length amyloid ?-proteins (1-40) and (1-42) in high-resolution structural studies. In contrast to the full-length amyloid ?-proteins, amyloid ?-protein (10-35) allowed the contro

[bAla8]-Neurokinin A(4-10)

B6813-10 10 mg
EUR 1479

Atholate™, protein hydrolysate, 10 kg

0134-10 10 kg
EUR 1982

Ethyl-β-D-glucuronide, 10 mg

0901-10 10 mg
EUR 554

Ethyl sulfate, sodium salt, 10 mg

0902-10 10 mg
EUR 132

Propyl-β-D-glucuronide, 10 mg

0903-10 10 mg
EUR 554

Human CellExp? IL-10, Human Recombinant

6466-10
EUR 343

Human IP-10 ELISPOT antibody pair

CT470-10 10-plate
EUR 528

Monkey IP-10 ELISA antibody pair

CT555-10 10-plate
EUR 547

Human IP-10 ELISA antibody pair

CT572-10 10-plate
EUR 547

Monkey IL-10 ELISPOT antibody pair

CT615-10 10-plate
EUR 528

Human IL-10 ELISPOT antibody pair

CT645-10 10-plate
EUR 528

Mouse IL-10 ELISPOT antibody pair

CT658-10 10-plate
EUR 528

Monkey IL-10 ELISA antibody pair

CT715-10 10-plate
EUR 547

Human IL-10 ELISA antibody pair

CT745-10 10-plate
EUR 547

Mouse IL-10 ELISA antibody pair

CT765-10 10-plate
EUR 547

MIP-1?/CCL9/10, murine recombinant

7174-10
EUR 207

10x17 cellogel 250µ (10)

EHCA1200-SH01-10 ea
EUR 56

10x17 cellogel 200µ (10)

EHCA1200-SH02-10 ea
EUR 56

10x17 cellogel 190µ (10)

EHCA1200-SH03-10 ea
EUR 56

10x17 cellogel 500µ (10)

EHCA1200-SH04-10 ea
EUR 69

16.5x14 cellogel 250µ (10)

EHCA1200-SH07-10 ea
EUR 69

16.5x14 cellogel 200µ (10)

EHCA1200-SH08-10 ea
EUR 69

16.5x14 cellogel 500µ (10)

EHCA1200-SH10-10 ea
EUR 78

17x17 cellogel 250µ (10)

EHCA1200-SH12-10 ea
EUR 87

17x17 cellogel 200µ (10)

EHCA1200-SH13-10 ea
EUR 87

17x17 cellogel 500µ (10)

EHCA1200-SH15-10 ea
EUR 105

20.5x20.5 cellogel 250µ (10)

EHCA1200-SH17-10 ea
EUR 214

20.5x20.5 cellogel 200µ (10)

EHCA1200-SH18-10 ea
EUR 214

20.5x20.5 cellogel 500µ (10)

EHCA1200-SH20-10 ea
EUR 260

30x30 cellogel 250µ (10)

EHCA1200-SH22-10 ea
EUR 433

30x30 cellogel 200µ (10)

EHCA1200-SH23-10 ea
EUR 433

30x30 cellogel 500µ (10)

EHCA1200-SH25-10 ea
EUR 496

18.3x14 cellogel 200µ (10)

EHCA1200-SH28-10 ea
EUR 141

18.3x17 cellogel 250µ (10)

EHCA1200-SH33-10 ea
EUR 169

AXYGEN® GEL TRAY 10 X 10 CM FOR USE WITH 10 CM GEL BOX, UV TRANSPARENT

HGB10-10-GT 1/pk
EUR 74
Description: Lab Equipment; Axygen Branded EQ

Ethyl-β-D-glucuronide-d5, 10 mg

0901D-10 10 mg
EUR 1562

Ethyl sulfate-d5, sodium salt, 10 mg

0902D-10 10 mg
EUR 244

2-Propyl-β-D-glucuronide, 10 mg

0904-10 10 mg
EUR 554

Ethyl-β-D-glucuronide-13C6, 10 mg

0905-10 10 mg
EUR 1473

CXCL10/IP-10/CRG-2, human recombinant

4277-10
EUR 185

DiagNano Silica-Coated Gold Nanoparticles, 10 nm

CG-10 10mL
EUR 719

AXYGEN® HORIZONTAL GEL BOX, 10 CM

HGB-10 1/pk
EUR 333
Description: Lab Equipment; Axygen Branded EQ

DiagNano Fluorophore Labeled Gold Nanoparticles, 10 nm

GFL-10 1 mL
EUR 1053

16.5x14 cellogel 190µ hr (10)

EHCA1200-SH09-10 ea
EUR 69

17x17 cellogel 190µ hr (10)

EHCA1200-SH14-10 ea
EUR 87

20.5x20.5 cellogel 190µ hr (10)

EHCA1200-SH19-10 ea
EUR 214

30x30 cellogel 190µ hr (10)

EHCA1200-SH24-10 ea
EUR 433

18.3x14 cellogel 190µ hr (10)

EHCA1200-SH27-10 ea
EUR 141

18.3x17 cellogel 190µ hr (10)

EHCA1200-SH32-10 ea
EUR 169

DiaEasy? Dialyzer (10, 15, 20 ml) Floating racks

1000-10
EUR 137

Ethyl-β-D-glucuronide-13C6-d5, 10 mg

0906-10 10 mg
EUR 2044

Human CellExp? Carbonic Anhydrase 10/CA10, human recombinant

7485-10
EUR 267

DiaEasy? Dialyzer (10 ml) MWCO 12-14 kDa

K1008-10
EUR 180

DiagNano Silica-Coated PEGylated Gold Nanoparticles, 10 nm

PG-10 10mL
EUR 719

Single channel mini-pipette (130 mm) 10 ul, autoclavable

SCMP-10 1
EUR 92

Saponin (Quillaja bark) pure, (sapogenin ?10%)

AV-4031-10 10 g
EUR 225

10 L RPMI 1640 WITH L-GLUTAMINE

10-040-LX 10 L/pk
EUR 206
Description: Media Catalog; Classical Media

TumorExoRNA? Tumor-derived exosome immunocapture and RNA extraction kit (10 reactions)

K1221-10
EUR 827

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 10 reactions)

M1030-10
EUR 838

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 10 reactions)

M1031-10
EUR 838

TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 10 reactions)

M1032-10
EUR 881

TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 10 reactions)

M1033-10
EUR 881

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 10 reactions)

M1034-10
EUR 838

MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 10 reactions)

M1035-10
EUR 838

TruStrip WBV Luciferase protein quantitation & Western Blot Validation Strips (10/pk)

WBLUC10-10 1 Pk
EUR 225

TruStrip WBV Tubulin protein quantitation & Western Blot Validation Strips (10/pk)

WBTUB10-10 1 Pk
EUR 225

AXYPREP MAG PLANT DNA-10 PREP

MAG-PLANT-gDNA-10 1/pk
EUR 72
Description: Bioscience Miscellaneous; Bioscience Miscellaneous - Axygen

TruStrip RDT Testosterone Rapid Test cards, 10/pk

1880-RDT-10 1 pk Ask for price

AXYPREP MAG TISSUE-BLOOD GDNA- SMALL - 10 PREPS

MAG-T-10 1/pk
EUR 50
Description: Bioscience Mag Beads; Magnetic Tissue GDNA

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 10 reactions)

M1036-10
EUR 838

MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 10 reactions)

M1037-10
EUR 838

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 10 reactions)

M1039-10
EUR 838

TruStrip WBV Flag-tag protein quantitation & Western Blot Validation Strips (10/pk)

WBFLA10-10 1 Pk
EUR 225

TruStrip WBV Green Fluorescent protein quantitation & Western Blot Validation Strips (10/pk)

WBGFP10-10 1 Pk
EUR 225

TruStrip WBV HA-tag protein quantitation & Western Blot Validation Strips (10/pk)

WBHAT10-10 1 Pk
EUR 225

Due to the complexity of preparing modRNA and the high cost of its reagents, there is a need for an improved, cost-efficient protocol to make modRNA. Here we show that changing the ratio between anti-reverse cap analog (ARCA) and N1-methyl-pseudouridine (N1mΨ), favoring ARCA over N1mΨ, significantly increases the yield per reaction, improves modRNA translation, and reduces its immunogenicity in vitro. This protocol will make modRNA preparation more accessible and financially affordable for basic and translational research.