High-throughput-compatible assays using a genetically-encoded calcium indicator.

Measurement of intracellular calcium in live cells is a key component of a wide range of basic life scienceresearch, and crucial for many high-throughput assays used in modern drug discovery. Synthetic calcium indicators have become the industry standard, due their ease of use, high reliability, wide dynamic range, and availability of a large variety of spectral and chemical properties.

Genetically-encoded calcium indicators (GECIs) have been optimized to the point where their performance rivals that of synthetic calcium indicators in many applications. Stable expression of a GECI has distinct advantages over synthetic calcium indicators in terms of reagent cost and simplification of the assay process.

Cortisol EIA Kit

EK7119 96wells/kit
EUR 478

Cortisol EIA Kit

SKT-201-96 1 plate of 96 wells
EUR 329
  • Cortisol, C21H30O5, (hydrocortisone, compound F) is the primary glucocorticoid produced and secreted by the adrenal cortex. It is often referred to as the ?stress hormone? as it is involved in the response to stress and it affects blood pressure, blo
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Description: Competitive EIA kit used for quantitative measuring cortisol present in Dried Fecal Samples, Saliva, Urine, Serum, EDTA Plasma, Heparin Plasma, Tissue Culture Media samples from all species

Corticosterone EIA Kit

SKT-205-96 1 plate of 96 wells
EUR 355
  • Corticosterone (C21H30O4, Kendall's Compound ?B') is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to stimulation of the adrenal cortex by ACTH and is the precursor of aldosterone. Corticosterone
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Description: Sandwich EIA kit used to measure the corticosterone present in Serum, EDTA Plasma, Heparin Plasma, Urine, Tissue Culture Media, Dried Fecal Samples samples from all species

DM1 ADC EIA Kit

DEIABL311 96T Ask for price
Description: This test kit is intended for use in the quantitative determination of antibody-DM1-conjugate level in test sample. It is useful for preclinical and clinical pharmacology study of DM1 Antibody Drug Conjugate (ADC). - Samples from tissue/cell culture a

MMAE ADC EIA Kit

DEIABL312 96T Ask for price
Description: This test kit is intended for use in the quantitative determination of antibody-MMAE-conjugate level in test sample. It is useful for preclinical and clinical pharmacology study of MMAE Antibody Drug Conjugate (ADC). - Samples from tissue/cell culture

MMAF ADC EIA Kit

DEIABL313 96T Ask for price
Description: This test kit is intended for use in the quantitative determination of antibody-MMAF-conjugate level in test sample. It is useful for preclinical and clinical pharmacology study of MMAF Antibody Drug Conjugate (ADC). - Samples from tissue/cell culture

Prostaglandin E2 EIA Kit

SKT-207-96 1 plate of 96 wells
EUR 527
  • Eicosanoid signal transduction pathways are highly conserved and are involved in a number of physiological processes. Prostaglandins are synthesized from arachidonic acid by cyclooxygenase (COX)-1 or -2, which convert the acid into PGH2. This is furt
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Description: Sandwich EIA kit used for quantitative measuring PGE2 present in Saliva, Urine, Serum, EDTA Plasma, Heparin Plasma, Tissue Culture Media samples from all species

Cyclic AMP EIA Kit

SKT-209-96 1 plate of 96 wells
EUR 454
  • Adenosine-3',5'-cyclic monophosphate (cyclic AMP) is a second messenger that plays an important role in intracellular regulation. Specifically, it functions as a mediator of activity of several key hormones including epinephrine, glucagon, and ACTH (
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Description: Direct EIA kit used for quantitative measuring cAMP present in Cell Lysates, Tissue, Saliva, Urine, EDTA Plasma, Heparin Plasma, Tissue Culture Media samples from all species

Cyclic GMP EIA Kit

SKT-211-96 1 plate of 96 wells
EUR 474
  • Guanosine 3', 5'-cyclic monophosphate (cyclic GMP
  • cGMP) is a critical and multifunctional second messenger present at levels typically 10-100 fold lower than cAMP in most tissues. Intracellular cGMP is formed by the action of the enzyme guanylate cy
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Description: Direct EIA kit used for quantitative measuring cGMP present in Cell Lysates, Tissue, Saliva, Urine, EDTA Plasma, Tissue Culture Media samples from all species

Cystatin C EIA Kit

SKT-219-96 1 plate of 96 wells
EUR 494
  • Cystatin C is a non-glycosylated protein of low molecular weight (13kDa) in the cystatin superfamily. Cystatin C is produced at a constant rate in all nucleated cells, secreted from cells and thus found in detectable amounts in most body fluids (1,2)
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Description: Sandwich EIA kit used to measure cystatin C levels in Serum, EDTA Plasma, Heparin Plasma, Urine, Tissue Culture Media samples from Human

EIA Wash Buffer 10x concentrate

1003-1 1 Liter
EUR 231

ImmunoComb Chlamydia Bivalent EIA kit

50416002 1 kit
EUR 370.6
Description: Please check the datasheet of ImmunoComb Chlamydia Bivalent EIA kit before using the test.

Androstenedione ELISA Kit (Competitive EIA)

EK7019 96wells/kit, with removable strips.
EUR 688

Aldosterone EIA Kit (One Plate)

K052-H1 1x96 well plate
EUR 387

Aldosterone EIA Kit (Five Plate)

K052-H5 5x96 well plate
EUR 1355

Estriol EIA Kit (One Plate)

K064-H1 1x96 well plate
EUR 327

Estriol EIA Kit (Five Plate)

K064-H5 5x96 well plate
EUR 1110

Allopregnanolone EIA Kit (One Plate)

K044-H1 1x96 well plate
EUR 425

Allopregnanolone EIA Kit (Five Plate)

K044-H5 5x96 well plate
EUR 1490

Insulin EIA Kit (One Plate)

K046-H1 1x96 well plate
EUR 501

Prolactin EIA kit (One Plate)

K040-H1 1x96 well plate
EUR 471

Oxytocin EIA Kit (One Plate)

K048-H1 1x96 well plate
EUR 450

Oxytocin EIA Kit (Five Plate)

K048-H5 5x96 well plate
EUR 1597

Epiandrosterone EIA Kit (One Plate)

K063-H1 1x96 well plate
EUR 436

Epiandrosterone EIA Kit (Five Plate)

K063-H5 5x96 well plate
EUR 1545

Estradiol EIA kit (One Plate)

K030-H1 1x96 well plate
EUR 283

Estradiol EIA kit (Five Plates)

K030-H5 5x96 well plate
EUR 985

Estrone EIA kit (One Plate)

K031-H1 1x96 well plate
EUR 300

Estrone EIA kit (Five Plates)

K031-H5 5x96 well plate
EUR 1001

Testosterone EIA kit (One Plate)

K032-H1 1x96 well plate
EUR 316

Testosterone EIA kit (Five Plates)

K032-H5 5x96 well plate
EUR 1045

Progesterone EIA kit (One Plate)

K025-H1 1x96 well plate
EUR 305

Progesterone EIA kit (Five Plates)

K025-H5 5x96 well plate
EUR 1012

Human Chlamydia pneumoniae IgG EIA Kit

DEIA1726 96T
EUR 1177
Description: CD's Chlamydia pneumoniae IgG test is developed for the detection of IgG antibodies specific to Chlamydia pneumoniae in human serum or plasma. The kit is semiquantitative allowing comparison of paired samples. The change of antibody level is an aid fo

Human Chlamydia pneumoniae IgM EIA Kit

DEIA1727 96T
EUR 1177
Description: Chlamydia pneumoniae IgM test (DEIA1727) is developed for the detection of IgM antibodies specific to Chlamydia pneumoniae in human serum or plasma. The positive result is an aid for the diagnosis of acute Chlamydia pneumonia infection. The test

Human Cotinine ELISA Kit (Competitive EIA)

EK7035 96wells/kit, with removable strips.
EUR 478

Human Opiates ELISA Kit (Competitive EIA)

EK7078 96wells/kit, with removable strips.
EUR 478

DetectX® Allopregnanolone EIA Antibody, 13ML

C226-13ML 13ML
EUR 1059

DetectX® Allopregnanolone EIA Antibody, 3ML

C226-3ML 3ML
EUR 298

DetectX® Epiandrosterone EIA Antibody, 13ML

C231-13ML 13ML
EUR 1044

DetectX® Epiandrosterone EIA Antibody, 3ML

C231-3ML 3ML
EUR 298

Resistin (human/mouse/rat) EIA Kit

K4767-100 100 assays
EUR 789
  • Kit components:
  • Resistin Microplate (Item A) coated with secondary antibody, 96 wells
  • Wash Buffer Concentrate (20x) (Item B)
  • Standard Resistin Peptide (Item C) (10 μl/vial)
  • Anti-Resistin polyclonal antibody (item N) (5 μl/vial)
  • Assay
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Description: Sensitive, Colorimetric Assay

Ghrelin (human, mouse, rat) EIA Kit

K4790-100 100 assays
EUR 789
  • Kit components:
  • Ghrelin Microplate (Item A) coated with secondary antibody, 96 wells
  • Wash Buffer Concentrate (20x) (Item B)
  • Lyophilized Standard Ghrelin Peptide (Item C)
  • Lyophilized anti-Ghrelin Polyclonal antibody (Item N)
  • 1 X Assay Dilue
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Description: Sensitive, Colorimetric Assay

Glucagon (human/mouse/rat) EIA Kit

K4756-100
EUR 843

Obestatin (human/mouse/rat) EIA Kit

K4759-100 100 assays
EUR 789
  • Kit components:
Description: Sensitive, Colorimetric Assay

17-Hydroxyprogesterone EIA kit (One Plate)

K053-H1 1x96 well plate
EUR 566

17-Hydroxyprogesterone EIA kit (Five Plate)

K053-H5 5x96 well plate
EUR 1980

DHEA-S EIA Kit (One Plate)

K054-H1 1x96 well plate
EUR 403

DHEA-S EIA Kit (Five Plate)

K054-H5 5x96 well plate
EUR 1436

ST2 Human EIA Kit, 1 Plate

K055-H1 1x96 well plate
EUR 593

Triiodothyronine (T3) EIA Kit (One Plate)

K056-H1 1x96 well plate
EUR 400

Triiodothyronine (T3) EIA Kit (Five Plate)

K056-H5 5x96 well plate
EUR 1394

Levonorgestrel (LNG) EIA Kit (One Plate)

K058-H1 1x96 well plate
EUR 557

Levonorgestrel (LNG) EIA Kit (Five Plate)

K058-H5 5x96 well plate
EUR 2080

20-Hydroxyecdysone EIA Kit (One Plate)

K066-H1 1x96 well plate
EUR 511

20-Hydroxyecdysone EIA Kit (Five Plate)

K066-H5 5x96 well plate
EUR 1953

Progesterone Metabolites EIA Kit (One Plate)

K068-H1 1x96 well plate
EUR 305

Progesterone Metabolites EIA Kit (Five Plate)

K068-H5 5x96 well plate
EUR 1012

Thyroxine (T4) EIA kit (One Plate)

K050-H1 1x96 well plate
EUR 400

Thyroxine (T4) EIA kit (Five Plates)

K050-H5 5x96 well plate
EUR 1394

Myeloperoxidase Human EIA Kit (One Plate)

K060-H1 1x96 well plate
EUR 516

DNA Damage EIA Kit (One Plate)

K059-H1 1x96 well plate
EUR 552

DNA Damage EIA Kit (Five Plate)

K059-H5 5x96 well plate
EUR 2074

Estradiol Serum EIA kit (One Plate)

KB30-H1 1x96 well plate
EUR 359

Estradiol Serum EIA kit (Five Plates)

KB30-H5 5x96 well plate
EUR 1246

Retinol Binding Protein Serum EIA Kit

K004-H1 1x96 well plate
EUR 318

Retinol Binding Protein Urinary EIA Kit

SKT-218-96 1 plate of 96 wells
EUR 527
  • Retinol binding protein (RBP) is from a family of structurally related proteins that bind small hydrophobic molecules such as bile pigments, steroids, odorants, etc (1). RBP is a 21 kDa highly conserved, single-chain glycoprotein, consisting of 182 a
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Description: Sandwich EIA kit used to measure RBP levels in urine samples in Urine samples from Human, Rat, Dog, Monkey

ANA IgG Screening ELISA Kit (Direct EIA)

EK7016 96wells/kit, with removable strips.
EUR 478

Human Brucella IgG ELISA Kit (Direct EIA)

EK7021 96wells/kit, with removable strips.
EUR 478

Human Brucella IgM ELISA Kit (Direct EIA)

EK7022 96wells/kit, with removable strips.
EUR 478

Human Cardiolipin IgG ELISA Kit (Direct EIA)

EK7024 96wells/kit, with removable strips.
EUR 478

Human Cardiolipin IgA ELISA Kit (Direct EIA)

EK7025 96wells/kit, with removable strips.
EUR 478

Human Cardiolipin IgM ELISA Kit (Direct EIA)

EK7026 96wells/kit, with removable strips.
EUR 478

Human DHEA-S ELISA Kit (Competitive EIA)

EK7047 96wells/kit, with removable strips.
EUR 478

Human Free Testosterone ELISA Kit (Competitive EIA)

EK7057 96wells/kit, with removable strips.
EUR 572

Human Morphine Specific ELISA Kit (Competitive EIA)

EK7070 96wells/kit, with removable strips.
EUR 478

Human Mumps IgG ELISA Kit (Direct EIA)

EK7073 96wells/kit, with removable strips.
EUR 478

Human Mumps IgM ELISA Kit (Direct EIA)

EK7074 96wells/kit, with removable strips.
EUR 478

Human Rubella IgG ELISA Kit (Direct EIA)

EK7082 96wells/kit, with removable strips.
EUR 478

Human Rubella IgM ELISA Kit (Direct EIA)

EK7083 96wells/kit, with removable strips.
EUR 478

Human Toxoplasma IgG ELISA Kit (Direct EIA)

EK7099 96wells/kit, with removable strips.
EUR 478

Human Toxoplasma IgA ELISA Kit (Direct EIA)

EK7100 96wells/kit, with removable strips.
EUR 478

Human Toxoplasma IgM ELISA Kit (Direct EIA)

EK7101 96wells/kit, with removable strips.
EUR 478

C-Peptide (human/mouse/rat) EIA Kit

K4757-100
EUR 843

Direct Cyclic GMP EIA Kit (One Plate)

K065-H1 1x96 well plate
EUR 369

Direct Cyclic GMP EIA Kit (Five Plate)

K065-H5 5x96 well plate
EUR 1293

PGE2 Multi-Format EIA kit (One Plate)

K051-H1 1x96 well plate
EUR 379

PGE2 Multi-Format EIA kit (Five Plate)

K051-H5 5x96 well plate
EUR 1344

Cyclic AMP Direct EIA Kit (One Plate)

K019-H1 1x96 well plate
EUR 369

Cyclic AMP Direct EIA Kit (Five Plate)

K019-H5 5x96 well plate
EUR 1293

Cyclic GMP Direct EIA kit (One Plate)

K020-H1 1x96 well plate
EUR 379

Cyclic GMP Direct EIA kit (Five Plate)

K020-H5 5x96 well plate
EUR 1369

Osteopontin Human (OPN) EIA kit (One Plate)

K021-H1 1x96 well plate
EUR 511

Human Cytomegalovirus (CMV) IgG ELISA Kit (Direct EIA)

EK7032 96wells/kit, with removable strips.
EUR 478

Human Cytomegalovirus (CMV) IgM ELISA Kit (Direct EIA)

EK7033 96wells/kit, with removable strips.
EUR 478

Human Chromogranin A (CgA) ELISA Kit (Direct EIA)

EK7034 96wells/kit, with removable strips.
EUR 688

Human Chlamydia Pneumoniae IgA ELISA Kit (Direct EIA)

EK7036 96wells/kit, with removable strips.
EUR 478

Human Chlamydia Pneumoniae IgG ELISA Kit (Direct EIA)

EK7037 96wells/kit, with removable strips.
EUR 478

We generated a clonal cell line constitutively expressing GCaMP6s; high expression of the GECI was driven by coupling to a blasticidin resistance gene with a self-cleaving cis-acting hydrolase element (CHYSEL) 2A peptide. Here, we compared the performance of the GECI GCaMP6s to the synthetic calcium indicator fluo-4 in a variety of assay formats. We demonstrate that the pharmacology of ion channel and GPCR ligands as determined using the two indicators is highly similar, and that GCaMP6s is viable as a direct replacement for a synthetic calcium indicator.