High-throughput-compatible assays using a genetically-encoded calcium indicator.

Measurement of intracellular calcium in live cells is a key component of a wide range of basic life scienceresearch, and crucial for many high-throughput assays used in modern drug discovery. Synthetic calcium indicators have become the industry standard, due their ease of use, high reliability, wide dynamic range, and availability of a large variety of spectral and chemical properties.

Genetically-encoded calcium indicators (GECIs) have been optimized to the point where their performance rivals that of synthetic calcium indicators in many applications. Stable expression of a GECI has distinct advantages over synthetic calcium indicators in terms of reagent cost and simplification of the assay process.

Cortisol EIA Kit
EK7119 96wells/kit
EUR 478

Prostaglandin E2 EIA Kit
SKT-207-96 1 plate of 96 wells
EUR 527
Description: Sandwich EIA kit used for quantitative measuring PGE2 present in Saliva, Urine, Serum, EDTA Plasma, Heparin Plasma, Tissue Culture Media samples from all species

Cyclic AMP EIA Kit
SKT-209-96 1 plate of 96 wells
EUR 454
Description: Direct EIA kit used for quantitative measuring cAMP present in Cell Lysates, Tissue, Saliva, Urine, EDTA Plasma, Heparin Plasma, Tissue Culture Media samples from all species

Cyclic GMP EIA Kit
SKT-211-96 1 plate of 96 wells
EUR 474
Description: Direct EIA kit used for quantitative measuring cGMP present in Cell Lysates, Tissue, Saliva, Urine, EDTA Plasma, Tissue Culture Media samples from all species

Cystatin C EIA Kit
SKT-219-96 1 plate of 96 wells
EUR 494
Description: Sandwich EIA kit used to measure cystatin C levels in Serum, EDTA Plasma, Heparin Plasma, Urine, Tissue Culture Media samples from Human

DM1 ADC EIA Kit
DEIABL311 96T Ask for price
Description: This test kit is intended for use in the quantitative determination of antibody-DM1-conjugate level in test sample. It is useful for preclinical and clinical pharmacology study of DM1 Antibody Drug Conjugate (ADC). - Samples from tissue/cell culture a

MMAE ADC EIA Kit
DEIABL312 96T Ask for price
Description: This test kit is intended for use in the quantitative determination of antibody-MMAE-conjugate level in test sample. It is useful for preclinical and clinical pharmacology study of MMAE Antibody Drug Conjugate (ADC). - Samples from tissue/cell culture

MMAF ADC EIA Kit
DEIABL313 96T Ask for price
Description: This test kit is intended for use in the quantitative determination of antibody-MMAF-conjugate level in test sample. It is useful for preclinical and clinical pharmacology study of MMAF Antibody Drug Conjugate (ADC). - Samples from tissue/cell culture

EIA Wash Buffer 10x concentrate
1003-1 1 Liter
EUR 231

Progesterone EIA kit (One Plate)
K025-H1 1x96 well plate
EUR 305

Progesterone EIA kit (Five Plates)
K025-H5 5x96 well plate
EUR 1012

Estradiol EIA kit (One Plate)
K030-H1 1x96 well plate
EUR 283

Estradiol EIA kit (Five Plates)
K030-H5 5x96 well plate
EUR 985

Estrone EIA kit (One Plate)
K031-H1 1x96 well plate
EUR 300

Estrone EIA kit (Five Plates)
K031-H5 5x96 well plate
EUR 1001

Testosterone EIA kit (One Plate)
K032-H1 1x96 well plate
EUR 316

Testosterone EIA kit (Five Plates)
K032-H5 5x96 well plate
EUR 1045

Prolactin EIA kit (One Plate)
K040-H1 1x96 well plate
EUR 471

Allopregnanolone EIA Kit (One Plate)
K044-H1 1x96 well plate
EUR 425

Allopregnanolone EIA Kit (Five Plate)
K044-H5 5x96 well plate
EUR 1490

Insulin EIA Kit (One Plate)
K046-H1 1x96 well plate
EUR 501

Oxytocin EIA Kit (One Plate)
K048-H1 1x96 well plate
EUR 450

Oxytocin EIA Kit (Five Plate)
K048-H5 5x96 well plate
EUR 1597

Aldosterone EIA Kit (One Plate)
K052-H1 1x96 well plate
EUR 387

Aldosterone EIA Kit (Five Plate)
K052-H5 5x96 well plate
EUR 1355

Epiandrosterone EIA Kit (One Plate)
K063-H1 1x96 well plate
EUR 436

Epiandrosterone EIA Kit (Five Plate)
K063-H5 5x96 well plate
EUR 1545

Estriol EIA Kit (One Plate)
K064-H1 1x96 well plate
EUR 327

Estriol EIA Kit (Five Plate)
K064-H5 5x96 well plate
EUR 1110

ImmunoComb Chlamydia Bivalent EIA kit
50416002 1 kit
EUR 370.6
Description: Please check the datasheet of ImmunoComb Chlamydia Bivalent EIA kit before using the test.

Androstenedione ELISA Kit (Competitive EIA)
EK7019 96wells/kit, with removable strips.
EUR 688

DetectX® Allopregnanolone EIA Antibody, 13ML
C226-13ML 13ML
EUR 1059

DetectX® Allopregnanolone EIA Antibody, 3ML
C226-3ML 3ML
EUR 298

DetectX® Epiandrosterone EIA Antibody, 13ML
C231-13ML 13ML
EUR 1044

DetectX® Epiandrosterone EIA Antibody, 3ML
C231-3ML 3ML
EUR 298

Retinol Binding Protein Serum EIA Kit
K004-H1 1x96 well plate
EUR 318

Thyroxine (T4) EIA kit (One Plate)
K050-H1 1x96 well plate
EUR 400

Thyroxine (T4) EIA kit (Five Plates)
K050-H5 5x96 well plate
EUR 1394

17-Hydroxyprogesterone EIA kit (One Plate)
K053-H1 1x96 well plate
EUR 566

17-Hydroxyprogesterone EIA kit (Five Plate)
K053-H5 5x96 well plate
EUR 1980

DHEA-S EIA Kit (One Plate)
K054-H1 1x96 well plate
EUR 403

DHEA-S EIA Kit (Five Plate)
K054-H5 5x96 well plate
EUR 1436

ST2 Human EIA Kit, 1 Plate
K055-H1 1x96 well plate
EUR 593

Triiodothyronine (T3) EIA Kit (One Plate)
K056-H1 1x96 well plate
EUR 400

Triiodothyronine (T3) EIA Kit (Five Plate)
K056-H5 5x96 well plate
EUR 1394

Levonorgestrel (LNG) EIA Kit (One Plate)
K058-H1 1x96 well plate
EUR 557

Levonorgestrel (LNG) EIA Kit (Five Plate)
K058-H5 5x96 well plate
EUR 2080

DNA Damage EIA Kit (One Plate)
K059-H1 1x96 well plate
EUR 552

DNA Damage EIA Kit (Five Plate)
K059-H5 5x96 well plate
EUR 2074

Myeloperoxidase Human EIA Kit (One Plate)
K060-H1 1x96 well plate
EUR 516

20-Hydroxyecdysone EIA Kit (One Plate)
K066-H1 1x96 well plate
EUR 511

20-Hydroxyecdysone EIA Kit (Five Plate)
K066-H5 5x96 well plate
EUR 1953

Progesterone Metabolites EIA Kit (One Plate)
K068-H1 1x96 well plate
EUR 305

Progesterone Metabolites EIA Kit (Five Plate)
K068-H5 5x96 well plate
EUR 1012

Estradiol Serum EIA kit (One Plate)
KB30-H1 1x96 well plate
EUR 359

Estradiol Serum EIA kit (Five Plates)
KB30-H5 5x96 well plate
EUR 1246

Resistin (human/mouse/rat) EIA Kit
K4767-100 100 assays
EUR 789
Description: Sensitive, Colorimetric Assay

Ghrelin (human, mouse, rat) EIA Kit
K4790-100 100 assays
EUR 789
Description: Sensitive, Colorimetric Assay

Glucagon (human/mouse/rat) EIA Kit
K4756-100
EUR 843

Obestatin (human/mouse/rat) EIA Kit
K4759-100 100 assays
EUR 789
Description: Sensitive, Colorimetric Assay

Retinol Binding Protein Urinary EIA Kit
SKT-218-96 1 plate of 96 wells
EUR 527
Description: Sandwich EIA kit used to measure RBP levels in urine samples in Urine samples from Human, Rat, Dog, Monkey

Human Cotinine ELISA Kit (Competitive EIA)
EK7035 96wells/kit, with removable strips.
EUR 478

Human Opiates ELISA Kit (Competitive EIA)
EK7078 96wells/kit, with removable strips.
EUR 478

Human Chlamydia pneumoniae IgG EIA Kit
DEIA1726 96T
EUR 1177
Description: CD's Chlamydia pneumoniae IgG test is developed for the detection of IgG antibodies specific to Chlamydia pneumoniae in human serum or plasma. The kit is semiquantitative allowing comparison of paired samples. The change of antibody level is an aid fo

Human Chlamydia pneumoniae IgM EIA Kit
DEIA1727 96T
EUR 1177
Description: Chlamydia pneumoniae IgM test (DEIA1727) is developed for the detection of IgM antibodies specific to Chlamydia pneumoniae in human serum or plasma. The positive result is an aid for the diagnosis of acute Chlamydia pneumonia infection. The test

Cyclic AMP Direct EIA Kit (One Plate)
K019-H1 1x96 well plate
EUR 369

Cyclic AMP Direct EIA Kit (Five Plate)
K019-H5 5x96 well plate
EUR 1293

Cyclic GMP Direct EIA kit (One Plate)
K020-H1 1x96 well plate
EUR 379

Cyclic GMP Direct EIA kit (Five Plate)
K020-H5 5x96 well plate
EUR 1369

Osteopontin Human (OPN) EIA kit (One Plate)
K021-H1 1x96 well plate
EUR 511

PGE2 Multi-Format EIA kit (One Plate)
K051-H1 1x96 well plate
EUR 379

PGE2 Multi-Format EIA kit (Five Plate)
K051-H5 5x96 well plate
EUR 1344

Direct Cyclic GMP EIA Kit (One Plate)
K065-H1 1x96 well plate
EUR 369

Direct Cyclic GMP EIA Kit (Five Plate)
K065-H5 5x96 well plate
EUR 1293

C-Peptide (human/mouse/rat) EIA Kit
K4757-100
EUR 843

ANA IgG Screening ELISA Kit (Direct EIA)
EK7016 96wells/kit, with removable strips.
EUR 478

Human Brucella IgG ELISA Kit (Direct EIA)
EK7021 96wells/kit, with removable strips.
EUR 478

Human Brucella IgM ELISA Kit (Direct EIA)
EK7022 96wells/kit, with removable strips.
EUR 478

Human Cardiolipin IgG ELISA Kit (Direct EIA)
EK7024 96wells/kit, with removable strips.
EUR 478

Human Cardiolipin IgA ELISA Kit (Direct EIA)
EK7025 96wells/kit, with removable strips.
EUR 478

Human Cardiolipin IgM ELISA Kit (Direct EIA)
EK7026 96wells/kit, with removable strips.
EUR 478

Human DHEA-S ELISA Kit (Competitive EIA)
EK7047 96wells/kit, with removable strips.
EUR 478

Human Free Testosterone ELISA Kit (Competitive EIA)
EK7057 96wells/kit, with removable strips.
EUR 572

Human Morphine Specific ELISA Kit (Competitive EIA)
EK7070 96wells/kit, with removable strips.
EUR 478

Human Mumps IgG ELISA Kit (Direct EIA)
EK7073 96wells/kit, with removable strips.
EUR 478

Human Mumps IgM ELISA Kit (Direct EIA)
EK7074 96wells/kit, with removable strips.
EUR 478

Human Rubella IgG ELISA Kit (Direct EIA)
EK7082 96wells/kit, with removable strips.
EUR 478

Human Rubella IgM ELISA Kit (Direct EIA)
EK7083 96wells/kit, with removable strips.
EUR 478

Human Toxoplasma IgG ELISA Kit (Direct EIA)
EK7099 96wells/kit, with removable strips.
EUR 478

Human Toxoplasma IgA ELISA Kit (Direct EIA)
EK7100 96wells/kit, with removable strips.
EUR 478

Human Toxoplasma IgM ELISA Kit (Direct EIA)
EK7101 96wells/kit, with removable strips.
EUR 478

Cyclic AMP Direct Chemiluminescent EIA kit (One Plate)
K019-C1 1x96 well plate
EUR 400

Cyclic AMP Direct Chemiluminescent EIA kit (Five Plate)
K019-C5 5x96 well plate
EUR 1471

Atrial Natriuretic Peptide (ANP) EIA kit (One Plate)
K026-H1 1x96 well plate
EUR 420

Atrial Natriuretic Peptide (ANP) EIA kit (Five Plates)
K026-H5 5x96 well plate
EUR 1394

Estrone-3-Glucuronide (E1G) EIA Kit (One Plate)
K036-H1 1x96 well plate
EUR 327

Estrone-3-Glucuronide (E1G) EIA Kit (Five Plate)
K036-H5 5x96 well plate
EUR 1088

Pregnanediol-3-Glucuronide (PDG) EIA Kit (One Plate)
K037-H1 1x96 well plate
EUR 321

Pregnanediol-3-Glucuronide (PDG) EIA Kit (Five Plate)
K037-H5 5x96 well plate
EUR 1061

Estrone-3-Sulfate (E1S) EIA Kit (One Plate)
K038-H1 1x96 well plate
EUR 533

Estrone-3-Sulfate (E1S) EIA Kit (Five Plate)
K038-H5 5x96 well plate
EUR 1980

Endothelin-1 (ET-1) EIA kit (One Plate)
K045-H1 1x96 well plate
EUR 557

Allopregnanolone EIA Kit, Monoclonal Antibody Based (One Plate)
K061-H1 1x96 well plate
EUR 425

Allopregnanolone EIA Kit, Monoclonal Antibody Based (Five Plate)
K061-H5 5x96 well plate
EUR 1490

Direct 2'3'-Cylic GAMP EIA Kit, (One Plate)
K067-H1 1x96 well plate
EUR 511

Direct 2'3'-Cylic GAMP EIA Kit, (Five Plate)
K067-H5 5x96 well plate
EUR 1953

Human Cytomegalovirus (CMV) IgG ELISA Kit (Direct EIA)
EK7032 96wells/kit, with removable strips.
EUR 478

Human Cytomegalovirus (CMV) IgM ELISA Kit (Direct EIA)
EK7033 96wells/kit, with removable strips.
EUR 478

Human Chromogranin A (CgA) ELISA Kit (Direct EIA)
EK7034 96wells/kit, with removable strips.
EUR 688

Human Chlamydia Pneumoniae IgA ELISA Kit (Direct EIA)
EK7036 96wells/kit, with removable strips.
EUR 478

Human Chlamydia Pneumoniae IgG ELISA Kit (Direct EIA)
EK7037 96wells/kit, with removable strips.
EUR 478

Human Chlamydia Pneumoniae IgM ELISA Kit (Direct EIA)
EK7038 96wells/kit, with removable strips.
EUR 478

Human Chlamydia Trachomatis IgG ELISA Kit (Direct EIA)
EK7041 96wells/kit, with removable strips.
EUR 478

Human Chlamydia Trachomatis IgM ELISA Kit (Direct EIA)
EK7042 96wells/kit, with removable strips.
EUR 478

Human Chlamydia Trachomatis IgA ELISA Kit (Direct EIA)
EK7043 96wells/kit, with removable strips.
EUR 478

Human Dengue Virus IgG ELISA Kit (Direct EIA)
EK7045 96wells/kit, with removable strips.
EUR 478

Human Dengue Virus IgM ELISA Kit (Direct EIA)
EK7046 96wells/kit, with removable strips.
EUR 478

Human HSV-1 IgG ELISA Kit (Direct EIA)
EK7058 96wells/kit, with removable strips.
EUR 478

Human HSV-1 IgM ELISA Kit (Direct EIA)
EK7059 96wells/kit, with removable strips.
EUR 478

Human HSV-2 IgG ELISA Kit (Direct EIA)
EK7060 96wells/kit, with removable strips.
EUR 478

Human HSV-2 IgM ELISA Kit (Direct EIA)
EK7061 96wells/kit, with removable strips.
EUR 478

Human Mycoplasma Pneumoniae IgG ELISA Kit (Direct EIA)
EK7071 96wells/kit, with removable strips.
EUR 478

Human Mycoplasma Pneumoniae IgM ELISA Kit (Direct EIA)
EK7072 96wells/kit, with removable strips.
EUR 478

Human Measles (Rubeola) IgG ELISA Kit (Direct EIA)
EK7075 96wells/kit, with removable strips.
EUR 478

Human Measles (Rubeola) IgM ELISA Kit (Direct EIA)
EK7076 96wells/kit, with removable strips.
EUR 478

Human Mitochondrial Antibody (MA) ELISA Kit (Direct EIA)
EK7077 96wells/kit, with removable strips.
EUR 478

Human SSA Autoantibody IgG ELISA Kit (Direct EIA)
EK7085 96wells/kit, with removable strips.
EUR 478

Human SSB Autoantibody IgG ELISA Kit (Direct EIA)
EK7086 96wells/kit, with removable strips.
EUR 478

Human Sm Autoantibody IgG ELISA Kit (Direct EIA)
EK7088 96wells/kit, with removable strips.
EUR 478

Human Salmonella Typhi IgM ELISA Kit (Direct EIA)
EK7089 96wells/kit, with removable strips.
EUR 478

Human Salmonella Typhi IgG ELISA Kit (Direct EIA)
EK7090 96wells/kit, with removable strips.
EUR 478

Human Thyroglobulin (TG) IgG ELISA Kit (Direct EIA)
EK7091 96wells/kit, with removable strips.
EUR 478

Human Treponema Pallidum IgG ELISA Kit (Direct EIA)
EK7095 96wells/kit, with removable strips.
EUR 478

Human Treponema Pallidum IgM ELISA Kit (Direct EIA)
EK7096 96wells/kit, with removable strips.
EUR 478

Alkaline Phosphatase (Calf, AP) Enzyme, purified EIA grade
ALP15-N-5 5 mg
EUR 286

Anthrax Protective Antigen IgG Screening ELISA Kit (Direct EIA)
EK7017 96wells/kit, with removable strips.
EUR 920

Human RNP Sm Autoantibody IgG ELISA Kit (Direct EIA)
EK7084 96wells/kit, with removable strips.
EUR 478

Human Scl-70 Autoantibody IgG ELISA Kit (Direct EIA)
EK7087 96wells/kit, with removable strips.
EUR 478

Human Thyroid Peroxidase (TPO) IgG ELISA Kit (Direct EIA)
EK7094 96wells/kit, with removable strips.
EUR 478

Human 25(OH) Vitamin D ELISA Kit (Competitive EIA)
EK7102 96wells/kit, with removable strips.
EUR 920

Retinol Binding Protein (RBP) Multi-format EIA Kit (One Plate)
K062-H1 1x96 well plate
EUR 369

Retinol Binding Protein (RBP) Multi-format EIA Kit (Five Plate)
K062-H5 5x96 well plate
EUR 1344

Retinol Binding Protein High Sensitivity EIA Kit (formerly named Urinary)
KU04-H1 1x96 well plate
EUR 410

Retinol Binding Protein High Sensitivity EIA Kit (formerly named Urinary)
KU04-H5 5x96 well plate
EUR 1491

Human Double Stranded DNA (dsDNA) IgG ELISA Kit (Direct EIA)
EK7044 96wells/kit, with removable strips.
EUR 478

Human Helicobacter pylori IgG ELISA Kit (Direct EIA) (H. pylori)
EK7064 96wells/kit, with removable strips.
EUR 478

Human Helicobacter pylori IgA ELISA Kit (Direct EIA) (H. pylori)
EK7065 96wells/kit, with removable strips.
EUR 478

Human Helicobacter pylori IgM ELISA Kit (Direct EIA) (H. pylori)
EK7066 96wells/kit, with removable strips.
EUR 478

Human VZV (Varicella zoster virus) IgG ELISA Kit (Direct EIA)
EK7103 96wells/kit, with removable strips.
EUR 478

Human VZV (Varicella zoster virus) IgM ELISA Kit (Direct EIA)
EK7104 96wells/kit, with removable strips.
EUR 478

Human Cardiolipin Total Ab (IgG, IgM, IgA) ELISA Kit (Direct EIA)
EK7027 96wells/kit, with removable strips.
EUR 478

Human EBV-VCA IgG ELISA Kit (Direct EIA) (Epstein-Barr Virus viral capsid antigen)
EK7050 96wells/kit, with removable strips.
EUR 478

Human EBV-VCA IgA ELISA Kit (Direct EIA) (Epstein-Barr Virus viral capsid antigen)
EK7051 96wells/kit, with removable strips.
EUR 478

Human EBV-VCA IgM ELISA Kit (Direct EIA) (Epstein-Barr Virus viral capsid antigen)
EK7052 96wells/kit, with removable strips.
EUR 478

We generated a clonal cell line constitutively expressing GCaMP6s; high expression of the GECI was driven by coupling to a blasticidin resistance gene with a self-cleaving cis-acting hydrolase element (CHYSEL) 2A peptide. Here, we compared the performance of the GECI GCaMP6s to the synthetic calcium indicator fluo-4 in a variety of assay formats. We demonstrate that the pharmacology of ion channel and GPCR ligands as determined using the two indicators is highly similar, and that GCaMP6s is viable as a direct replacement for a synthetic calcium indicator.